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1. Before you start...
- Consult peers and evaluate protocol
- Legal requirements
- Minimize animal handling
- Know your animal
- Use technology and cutting edge resources
- Know your handling procedure
- Know your equipment
- Record procedure on field forms
- Prepare ahead for the difficult issues
- Euthanasia
- Non-target species captures
- Media
1.1. Preliminary Planning
- Purpose of capture
- Type of terrain or escape cover
- Type of animal
- Condition of animal
- Emotional state of animal
- Safety equipment
- Temperature affecting animal
- Time of day
- Appropriateness of drugs
- Physical or chemical restraint
- Available assistance
2. Mechanical Restraint
The person capturing or administering drugs to a wild animal assumes the responsibility for the life of that animal!
2.1. Prior to capture
- Purpose - is it justified?
- Type of animal
- Type of terrain or escape cover
- Condition of animal
- Emotional state of the animal
- Safety equipment available
- Temperature affecting the animal
- Time of day
- Which drug(s) would be most effective?
- Would only physical restraint be better?
- Do you have assistance and professional support?
2.2. Physical Restraint Equipment
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![]() Physical restraint only is used to capture and
transport Big Horn sheep in California
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2.2.1. Snare Poles
- Any rope, cable, wire or monofilament
- Pipe or pole
- From small mammals and birds to rhinos
- Ketch-All Pole
- Whitney Restraint Pole
- Graspers
2.2.2. Snares
- Less impact than leghold traps
- Aldrich foot snares- black bears
- Lembeck leg snare- for foxes, bobcats and coyotes
- Foot snares- ungulates
- Ashcraft snare- migrating deer
- Bal-Chatri or hoop (Phi Trap)- raptors
- Neck snares- coyotes and other carnivores
2.2.3. Leghold or Gin Traps
- 'Soft-Catch' version
- 'Tranquilizer tabs' available
- Purse-Jawed traps/Bailey or Hancock traps - aquatic mammals
2.2.4. Traps
- National Live Trap
- Tomahawk
- Havahart
- Sure Catch
- Sherman Traps
- Barrel Traps (Rudolph traps)- small carnivores
- Culvert traps- bears
- Large box traps/giant "Havahart-style"- wild pigs, deer
- Clover Traps- deer, elk
- Panel Traps
- Corral Traps
- Trapsite transmitters
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3. Chemical Restraint
3.1. Background
3.1.2. The Age of discovery - 1850's-1950
- Alpha-chloralose
- Chloral hydrate
- Chloroform
- Ether
- Sodium pentobarbital
- IV, IP, IM, Oral
3.1.3. The Paralytic era - 50's and 60's
- Paraldehyde
- Curare
- Nicotine
- Succinyl choline
- Flaxidil on a drill
3.1.6. The 90's and beyond
- Age of designer neurophamaceuticals
- Targeted agonists and antagonists
- Long acting neuroleptics
| The Perfect Drug | |
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Safe Low volume Reversible Rapid acting Anxiolytic and analgesic |
NO side effects Non-narcotic Approved for food animals Inexpensive No human safety hazard |
3.2. Legal Dimensions
- Need FDA approval & License from DEA
- Extra-label use
- Experimental drugs under Investigational New Animal Drug File (INAD)
3.2.1. Drug Schedules
[II-IV Substances have decreasing abuse liabilities (II is the highest) and approved medical use]
3.2.1.1. I
Substances have a high abuse liability and no approved medical use
- Heroin, LSD, THC, marijuana, peyote, mescaline
3.2.1.3. Special II
Potent narcotics and short-acting barbiturates.
- Etorphine/Diprenorphine, Carfentanil, Fentanyl/Droperidol
3.3. Preparation for Chemical Restraint
Successful anesthesia ends with careful planning
- Environment
- Equipment
- Expectations
- Evaluation of drugs
3.3.1. Environmental factors
- Distance/Dart/Drug/Damage equation
- Terrain - fences, falls, water, visibility
- Calendar and clock - time of year/time of day
3.3.2. Drug Delivery Systems
- Syringe Pole (jab stick)
- Dart Delivery Systems
- Internal powder charges
- Palmer Cap-Chur Equipment
- Pneu-Dart




- Air-pressured Charges
- Distinject Injectors
- Dan-Inject
- Telinject
- Blow Pipe
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3.4. The Drugs
3.4.1. Opioids
| Opioids | Opioid Antagonists |
| Etorphine (M-99) | Diprenorphine HCL (M50-50) |
| Carfentanil | Naloxone |
| A3080 | Naltrexone* |
| Nalmafene |
- Central nervous system depression
- Used alone or in combination with neuroleptics to avoid excitement
- Rapid induction
- Wide safety margin, predictable
- Good analgesic properties
- Reversible
- Low dose volume
- Controlled narcotics
- Expensive
3.4.1.1. Clinical Signs
- Ataxia, incoordination
- High stepping
- Visual impairment
- Loss of fear of humans or objects
- Animal may stay on feet or collapse
3.4.1.2. Side Effects
- Excitation, running, pacing, walking
- Vomiting
- Respiratory depression
- Muscle tremors and rigidity
- Hypertension, hypotension
- Hyperthermia, hypothermia
3.4.1.3. Disadvantages
- Toxic to humans at low doses
- Major respiratory depressants
- Re-narcotization after antagonism
- Prolonged, excitatory state prior to induction
- Alter thermoregulation
- Poor muscle relaxation
3.4.1.4. Etorphine (M-99)
- 2000-4000 x morphine
- Semisynthetic morphine
- Combined with α2's
- Large experience base in the field
- Species and efficacy range widely known
3.4.2. Alpha-Two Agonists
| Alpha-Two Agonists | Alpha-Two Antagonists |
| Xylazine | Yohimbine (Yobine, Antagonil) |
| Detomidine | Tolazoline (Tolazine) |
| Medetomidine | Atipamezole (Antisedan)* |
- Central nervous system depressant with sedative, muscle relaxant, and analgesic properties
- Ruminants, equids, suids, carnivores,birds
- Xylazine, Detomidine (2-3x) Medetomidine (10x)
- Combination with Ketamine or Opioids
3.4.2.1. Advantages
- Non-controlled
- Inexpensive
- Rapid absorption
- Analgesia
- Good muscle relaxation
- Reversible
3.4.2.2. Disadvantages
- Initial hypertension followed by severe hypotension
- Bradycardia, AV block
- Prolonged effect if not antagonized
- Hyperglycemia and glucosuria
- Disrupts thermoregulation
- Regurgitation in carnivores
3.4.3. Dissociatives (Cyclohexamines)
- Carnivores, Primates, Birds
- Negative effects are preventable with concurrent administration of tranquilizers
- Ketamine - often used with α-2s, benzodiazepines, or acepromazine
- Telazol - Tiletamine & Zolazepam advantage over Ketamine is its solubility and 2.5 times more potent but is not reversible
3.4.4. Clinical Signs
- Multiple sites of action in CNS
- Dysphoria
- Salivation
- Head weaving
- Wide eyes, Dilated pupils
- Recumbency
4. CAPTURE EMERGENCIES
4.1. General Hazards
- Trauma
- Capture wounds (bandaged animals cannot be released)
- Contusions
- Abrasions
- Lacerations
- Fractures: necks and legs
- Abortions
- Malpositions
- Tympanism
- Asphyxiation
- Seizures
- Overdose
- Renarcotization
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4.2. Respiratory Depression or Arrest
Definition: Tissue hypoxia resulting in necrosis or damage caused by inadequate oxygenation of blood hemoglobin
4.2.1. Causes
- Drug-induced depression of respiratory center
- Airway obstruction (nose/trunk/tracheal occlusion, edema/vomiting blocking airway)
- Pressure of the diaphragm (bloat, intestinal contents)
4.2.2. Clinical Signs
- Few or no respirations
- Cyanosis
- Noisy breathing
- Oxygen saturation <70% for more than 2 min SO2
4.3. Hyperthermia
4.3.1. Causes
- Metabolic heat from physical exertion
- Heat absorption from environment (direct exposure to sun, confinement in poorly ventilated space)
- Drug-induced alteration of thermoregulatory centers
- Bacterial infection
4.3.2. Treatment
- Cease all further administration of immobilizing drugs
- Cool the animal (move out of direct sunlight)
- Whole body immersion: most effective
- Spray entire animal (groin & belly)
- Pack ice or cold water bags on groin, head
- Douse with isopropyl alcohol (rapid evaporation cools quicker)
- Cold water edema
- Cold lactated Ringer's solution IV or IP
- Administer appropriate antagonist IV
4.4. Hypothermia/Frostbite
4.4.1. Causes
- Drug-induced (decreased metabolism/endogenous heat production; alteration of thermoregulatory center
- Cold ambient temperature
- Loss of insulation (wet, soaked coat)
- Oiled fur or feathers
- Malnutrition (decreased fat)
- Long recumbency (compression)
- Inadequate circulation (shock, foothold trap)
4.5. Shock
Definition: Clinical syndrome characterized by ineffective blood perfusion of tissues resulting in cellular hypoxia. It is often seen in animals that underwent a stressful or strenuous capture.
4.5.1. Causes
- Prolonged physical exertion
- Prolonged physiological/psychological stress
- Severe blood loss
4.5.2. Clinical Signs
- Rapid heart rate
- Low blood pressure (slow capillary refill)
- Muscle weakness
- Depressed sensorium (masked by drugs)
- Hyperventilation
4.6. Bloat/Tympanism
Treatment
- Correct body position (sternal or right recumbency)
- Pass stomach tube (48" long x 5/8" OD) - lubricate first, be sure not in trachea!!
- Insert large-bore needle into left flank to release gas 4. Administer appropriate antagonist drug
4.7. Aspiration/Vomiting
4.7.2. Clinical Signs
- Gurgling sounds during respiration
- Choking, gasping
- Cyanosis
- Presence of foreign material in larynx, trachea
- Respiratory arrest
4.8. Seizures/Convulsions
Definition: Transient disturbance of cerebral function characterized by a violent, involuntary contraction or series of contractions of the voluntary muscles
4.8.2. Clinical Signs
- Uncontrolled muscle spasms- whole body spasms
- Rigid extension of limbs
- Mouth gaping
4.9. Cardiac Arrest
Definition: Loss of effective cardiac function resulting in cessation of circulation
4.9.1. Causes
- Drug-induced
- Hypoxia -respiratory failure
- Acid-base imbalance
- Electrolyte imbalance - hyper/hypokalemia, hypocalcemia
- Autonomic nervous system imbalance - increased sympathetic/ parasympathetic tone
- Hypothermia
4.9.2. Clinical signs
- Weak or absent heart sounds or pulse
- Poor capillary refill (> 2 sec)
- Cyanosis
- Increased respiratory rate, abnormal pattern or apnea
- Dilated pupils
- Cold skin
- Loss of consciousness
4.9.3. Treatment
- Cease all further administration of immobilizing drugs
- Make sure that the animal can breathe -artificial respiration/doxapram
- Begin external cardiac massage - press at one release at one with 60-100 cycles/min - detect femoral pulse
- Inject 0.2 mg/kg of 1:10,000 epinephrine IV or IC and continue massage. Insert needle between 4th and 6th ribs, above and behind elbow. NOTE: many epinephrine concentrations come as 1:1,000; dilute each ml with 9 ml of physiological saline or lactated Ringer's
- If no response to the above, inject 0.1 ml/kg calcium chloride solution (10% or 100 mg/ml) IV or IC
- If still no response, repeat epinephrine and calcium chloride doses plus inject 10-20 mEq sodium bicarbonate IV or IC
4.9.4. Comments
- CRT is method to assess peripheral perfusion and cardiac function
- This is just a field method to Dx cardiac arrest- in practice a very complicated situation
- Many cardiac arrests arrive from metabolic disturbances due to physical exertion or stress
- Need ECG- one available for field situations
4.10. Dehydration
4.10.1. Causes
- Decreased water intake
- Hyperthermia- loss by transpiration
- Fever
- Chronic vomiting
- Wound drainage
- Polyuria
4.10.2. Clinical Signs
- Skin lacks pliability
- Mouth, gums dry
- Weak pulse
- Depressed sensorium
- Signs of shock
4.10.3. Treatment
- Cease all further administration of immobilizing drugs
- Determine the volume deficit (4-6-8-10 rule)
- Loss of 4% in body weight is determine by fluid loss or history
- Loss of 6% obvious fluid deficits- mouth mucosa red and dry
- Loss of 8% severe fluid loss - weak pulse, depression
- Loss of 10% life threatening- shock
- Administer fluid therapy
4.11. Capture Myopathy
Definition: CM is a complex condition affecting animals undergone a stressful or strenuous capture or handling. The pathophysiology of CM is complex (see Williams & Thorne, 1998).
4.11.3. Treatment
Administer 5 meq/kg sodium bicarbonate IV. Administer slowly (4-5 ml/min) to avoid cardiac arrhythmias
4.11.4. Comments
- Pathogenesis not completely understood, treatment difficult and unsuccessful
- Lactic acidosis a consistent finding
- Restoration of normal pH in blood is thought to ameliorate pathology
- Clinical signs may appear in hours (acute form) or may take several days to develop (ataxic-myoglobinuric/rupture muscle syndromes)
- Serum chemistries severely altered
- CM reported in all animal groups but more common in ungulates
- Vitamin E and Selenium deficiency may be a predisposing factor
4.12. HUMAN SAFETY
4.12.1. Human Exposure to Chemical Restraint Agents
- Cyclohexamines - Behavioral toxicity
- Xylazine - Respiratory support
- Opioids - Coma and possible death Naltrexone IV
4.13. EUTHANASIA
- Plan ahead
- AVMA Euthanasia Standards http://www.avma.org/resources/euthanasia.pdf
- IV Barbiturates
- CO2 or CO
- Penetrating captive bolt pistol
- Gunshot
5. Ancillary Material
5.1. Readings
5.1.1. Texts and Articles
The Capture and Care Manual : capture, care, accommodation and transportation of wild African animals. ed. by Andrew A. McKenzie. Wildlife Decision Support Services CC. 1993.
Clark, R. K. and D. A. Jessup (eds.). 1992. Wildlife Restraint Series. International Wildlife Veterinary Services, Inc., Salinas, California.
Fowler, Murray E. Restraint and handling of Wild and Domestic Animals. 2nd ed. Iowa State University Press. 1995.
Haigh, Jerry, C. Opioids in zoological medicine. Journal of Zoo and Wildlife Medicine, V. 21 (4), 1990, pp. 391-413.
Jalanka, Harry, H. the use of medetomidine, medetomidine-ketamine combinations, and atipamezole in nondomestic mammals: a review. Journal of Zoo and Wildlife Medicine, v. 21 (3), 1990, pp. 259-282.
Kreeger, Terry J. Handbook of Wildlife Chemical Immobilization. International Wildlife Veterinary Services. 1996.
Nielsen, Leon. Chemical Immobilization of Wild and Exotic Animals. Iowa State University Press, 1999.
Williams, Elizabeth S. and E. Thomas Thorne. Exertional myopathy (capture myopathy). Chapter 16 In Noninfectious Diseases of Wildlife. A. Fairbrother, et al. (ed.). Iowa State University Press, 1998.
5.2. Websites
US Drug Enforcement Agency (incl. Schedule of Controlled Substances) http://www.usdoj.gov/dea/index.htm
Zoological Restraint and Anesthesia, Heard D. (Ed.). an online text in the IVIS Library http://www.ivis.org/
5.3. Advanced Courses in Restraint
International Wildlife Veterinary Services, 1850 N. Main, Salinas, CA 93906 Wildlife Veterinary Resources, Mark R. Johnson, DVM, (406) 586-4624 wildlifevet@gomontana.com
Safe-Capture International, Keith Amass (608) 767-3071 www.safecapture.com











