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Stain:

Masson Trichrome

Description:

Section of pancreas showing two profiles of interlobular ducts (center) surrounded by exocrine acinar tissue. Often referred to as a connective tissue stain, Masson trichrome is one of several "polychrome" procedures, which have been developed using multiple dyes to differentiate tissues by color. In this preparation, collagen of the interlobular CT is staining bright blue (due to acid dye aniline blue) in contrast to the cytoplasmic reds (due to other acid dyes) of the epithelial glands. The ergastoplasm, which stains with the nuclear (basic) dye, appears purple due to the blue-plus-red combination in those portions of the cytoplasm. The nuclei that appear to be "floating" in the center of acini (and that are not surrounded by ergastoplasm) belong to centroacinar cells. (ag01)

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