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PREV : F1'-2:Lymphocyte, Blood Smear
Author: Alvar W Gustafson, Ph.D.
F1'-12:Basophil, Blood Smear
Stain:Wright's
Description:Blood smear showing a basophil and surrounding erythrocytes. The prominent granules in the basophil stain metachromatically with the methylene blue/azure components of the dye mixture. This staining reaction, which typically obscures the nucleus, is due to the presence of proteoglycans and the GAG heparin--polyanions that are contained within the specific granules. The erythrocytes stain orthochromatically with eosin. Note the "central pallor" that is seen in singular erythrocytes. What does this staining appearance suggest about the shape of these cells? In normal blood, basophils are the least abundant of all leukocytes. (ag01)